For my research, I would like to preserve some strawberry leaves for a few weeks to analyze nutrients and nitrogen levels and take DNA samples. However, my research focuses on different sources and concentrations of nitrogen on strawberry plant growth, where I'm concerned that liquid nitrogen might alter this data. Is there any alternative method available for long-term preservation of leaves that won't affect DNA and nutrient analysis? Thanks!
For nutrient analysis , you can store dried and well ground leaves at room temperature , no issue..But for DNA analysis , off course , samples should be stored as suggested above..
you can store your leaves in -20 or -80 C freezer but it will not store for long. For nutrients dry and store in airtight container. For DNA analysis its better to extract from fresh leaves or parts and store it in -20.
For DNA analysis, clean the surface of the leaves with distill water and then ethanol and store it in -20 degree C. You can store for couple of weeks in this way. Use separate batch of leaves for DNA analysis and nutrient analysis.
For DNA extraction fresh leaves is always better but You can keep it in -20 degree C freezer also.