Can anyone help me to remove a hydrophobic peptide from C18 column?

I have injected a hydrophobic peptide into analytical C18 column and i have tried to cleaning the column applying a lot of protocols using acetonitrile, methanol, isopropanol, but in the blank...

05 January 2021 3,305 3 View

Could you help with a doubt regarding UDF velocity inlet profile with multiphase mixture (Ansys Fluent)?

We are two undergraduate students seeking for help!!! We are trying to simulate a laminar flux through a 3D pipe (25 cm long, 2 cm of diameter) consisting of a multiphase flux (air and water). We...

23 November 2020 3,918 3 View

What is the role of Digital Services in Crisis, Disaster, and Emergency Situations?

06 January 2020 7,241 4 View

How to dissolve Ibuprofen?

I need to obtain IBU aqueous solution. Some papers report that they dissolve it in water/methanol (90/10). But it did not work. What to use to dissolve it? Some buffer or NaOH? Or maybe some salt....

05 May 2019 6,575 1 View

How come conductivity increased at very low concentrations of CuSO4 after sorption of activated carbon?

I was investigating the sorption of activated carbon on different concentrations of copper(II)sulfate and came across rather unexpected results where the conductivity increased at 0,00001 , 0,0001...

24 November 2018 6,855 3 View

It's been said that DNA sequencing is semi-quantitative. What does this mean? And what evidence is there?

The interpretation of most microbial meta-barcoding studies hinge on the behavior of the sequencing platforms. And, it seems to me that there is a philosophical divide amongst microbial...

25 May 2018 3,718 8 View

Can I use aneuploidy for crossing?

Dear Collegues, we are going to make segregating population of tea plant (camellia sinensis) for genetic research. we need 200 progenies. Can we use aneuploid cultivar as one of the parents to...

19 October 2017 8,451 3 View

Protocol of fungal dna extraction from plant leaf tissue?

Dear collegues, Can we use protocols of DNA extraction from plant tissues (from leaves) to extract DNA of fungal pathogen which lives inside of tissue? If yes, will the quantity of the pathogen's...

11 April 2017 8,448 31 View

If I have low bootstrap values in a ML tree, does it mean that there is no separation between different genera?

I have a maximum likelihood tree (18S and COI) for a nematode family which contains 4 different genera. The bootstrap values were very low and all the 4 genera are intermingled in both trees with...

27 June 2016 593 5 View

Why does the PCR not work occasionally?

Dear collegues! we extracted RNA from apple peel. We need clean RNA without DNA contamination, so we check our RNA samples by PCR. Samples are fresh. and reagents are the same. BUT one time PCR...

24 March 2015 4,855 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View