I am currently maintaining a Drosophila cell line. The media is supplemented with Pen-Strep and Amphotericin B.
Typically, the Amphotericin B is well inhibits the growth of fungi with minor contamination and subsequent routine passage there is a gradual deliverance from the culture. If a massive infection is usually easier to get rid of, maybe (especially in the case of yeast) often there is resistance to antimycotic. If the culture is rare (e.g. producer of something, or the result of some experiment) - sometimes switching incubation at high sublethal doses (for each cell line is its own and is sought experimentally) from several hours to 1-2 days, followed by lowering the concentration to non-toxic.
Hi Ernest,
I would go with Amphotericin B since it works good in many cultures.
Best,
Milica
Hi Ernest,
Nystatin is a great anti-fungal drug, although I haven't tried it with the Drosophila cell line. I've also read that Amphotericin B works as well.
Good luck.
NRB
Thank you for all your suggestions to my inquiry. Fortunately, we have already traced the source of contamination and have eliminated it.
Thank you.
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