Does anyone have a general Hydrophobic Interaction Chromatography Protocol?

I have a sample suspended in 0.34 M NaCl after using ion exchange chromatography. I would like to perform hydrophobic interaction chromatography (HIC). Is it okay that I use greater salt...

07 August 2019 9,739 1 View

What is the difference between the columns Hi Prep 26/60 S-100 HR and Hi Prep 26/60 S-200 HR?

I am trying to have a better resolution during purification. Would running my sample using S-100 HR give a better result from an S-200 HR run? Also, other than the fractionation range, what is...

06 July 2019 672 4 View

Does anyone know the concentration of antibiotics used in culturing stably transfected Drosophila S2 cells?

I am culturing transfected Drosophila S2 cells. I would like to culture them into a serum-free media (SFM). However, I have read from a paper that cells cultured in SFM are more sensitive to...

02 March 2018 8,864 5 View

Does anyone observe decrease in growth rate of Drosophila S2 cells in serum-free media?

I have adapted my Drosophila S2 cells growing in complete Schneider's medium to serum-free media. After a month of passage in serum-free media, the cells continually exhibit decreased growth rate...

11 December 2017 145 3 View

Does anyone know how to measure osmotic pressure in a Schneider's Drosophila medium?

06 July 2017 513 2 View

Does anyone know the recommended/required amount of antibody needed to label a PVDF membrane (from a 0.75 mm, 10 well gel)?

I did a western blot with a PVDF membrane. Because I only have 5 samples, I have thought of using 5 ml (1:1000 antibody dilution) of antibodies (Ni-NTA AP Conjugate). Unfortunately, I have...

06 July 2017 6,649 3 View

What is a good antimycotic drug that can be used to eliminate fungal contamination in a Drosophila cell culture?

I am currently maintaining a Drosophila cell line. The media is supplemented with Pen-Strep and Amphotericin B.

02 March 2017 5,030 4 View

Does anyone know if MALDI-TOF MS can be used to identify protein structure?

I am unfamiliar to how MALDI-TOF MS can identify protein structure. I have read websites indicating that it can be done. However, I would like to know of how accurate are the results of MALDI-TOF...

01 January 1970 8,839 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View