Ernest Troyss Pilapil

9 Questions 12 Answers 0 Followers

Questions related from Ernest Troyss Pilapil

Does anyone have a general Hydrophobic Interaction Chromatography Protocol?

I have a sample suspended in 0.34 M NaCl after using ion exchange chromatography. I would like to perform hydrophobic interaction chromatography (HIC). Is it okay that I use greater salt...

08 August 2019 9,825 1 View

What is the difference between the columns Hi Prep 26/60 S-100 HR and Hi Prep 26/60 S-200 HR?

I am trying to have a better resolution during purification. Would running my sample using S-100 HR give a better result from an S-200 HR run? Also, other than the fractionation range, what is...

07 July 2019 778 4 View

Does anyone know the concentration of antibiotics used in culturing stably transfected Drosophila S2 cells?

I am culturing transfected Drosophila S2 cells. I would like to culture them into a serum-free media (SFM). However, I have read from a paper that cells cultured in SFM are more sensitive to...

03 March 2018 8,949 5 View

Does anyone observe decrease in growth rate of Drosophila S2 cells in serum-free media?

I have adapted my Drosophila S2 cells growing in complete Schneider's medium to serum-free media. After a month of passage in serum-free media, the cells continually exhibit decreased growth rate...

12 December 2017 227 3 View

Does anyone know a protocol for extracting plasmids in Drosophila S2 cells?

If I perform a genomic DNA extraction of the S2 cells, would the plasmid DNA be included too with the DNA extracts? Also, would there be any consequences if I perform a PCR amplification of the...

10 October 2017 2,975 2 View

Does anyone know the recommended/required amount of antibody needed to label a PVDF membrane (from a 0.75 mm, 10 well gel)?

I did a western blot with a PVDF membrane. Because I only have 5 samples, I have thought of using 5 ml (1:1000 antibody dilution) of antibodies (Ni-NTA AP Conjugate). Unfortunately, I have...

07 July 2017 6,754 3 View

Does anyone know how to measure osmotic pressure in a Schneider's Drosophila medium?

I am about to reconstitute a Schneider's powdered medium from Sigma. I have read their protocol that says in step 10, osmotic pressure of 360 mOsm is suitable for the growth of the Drosophila...

07 July 2017 621 2 View

What is a good antimycotic drug that can be used to eliminate fungal contamination in a Drosophila cell culture?

I am currently maintaining a Drosophila cell line. The media is supplemented with Pen-Strep and Amphotericin B.

03 March 2017 5,111 4 View

Does anyone know if MALDI-TOF MS can be used to identify protein structure?

I am unfamiliar to how MALDI-TOF MS can identify protein structure. I have read websites indicating that it can be done. However, I would like to know of how accurate are the results of MALDI-TOF...

01 January 1970 8,930 4 View