Hello,
Usually, in my lab used only pET-D topo 100 vector for protein expression (T7 promoter). Now, I plan to express my target gene. The target gene is inserted in pQE9 (T5 pomoter; Qiagen). So, I want to know the different between T5 and T7 promoter.
Thanks
They are two different DNA sequences recognized by two different enzymes. T7 requires coexpression of T7 RNA polymerase (such as a DE3 cell line). While the T5 promoter can be recognized by bacterial RNA polymerase and doesn't require T7 expression.
They are two different DNA sequences recognized by two different enzymes. T7 requires coexpression of T7 RNA polymerase (such as a DE3 cell line). While the T5 promoter can be recognized by bacterial RNA polymerase and doesn't require T7 expression.
Thanks, Mr. Dunn
From my experience, T7 showed very strong promoter to provide the high expression level. Whether T5 also strong promoter to produce a high expression?
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