7 Questions 5 Answers 0 Followers
Questions related from Sarit Charuchaibovorn
Hello, Usually, in my lab used only pET-D topo 100 vector for protein expression (T7 promoter). Now, I plan to express my target gene. The target gene is inserted in pQE9 (T5 pomoter; Qiagen)....
02 February 2016 8,097 4 View
Hi, researcher As you know, It's hard to study about everything of the parasite also an interaction between hosts (protein, substance) and parasite in the molecular levels. So, I want to know is...
12 December 2015 939 1 View
Hi,I want to check gene sequence that is a large size (3.7kb). This gene is located in MCS of pQE9 vector (Qiagen). How many primers that i should design for check full gene ?Do i need to...
03 March 2015 8,743 6 View
How can I determine the IPTG concentration for difference strains of E.coli For example, I found in a research paper for protein expression1. protein in expression vector (T5 promoter) in E.coli...
12 December 2014 1,788 3 View
I want to amplify the uncharacterized gene. First step, I will aplify and sequencing full cDNA of gene.What is the best (quality and price) the RACE kit ? (First choice, invitrogen and SMARTER...
10 October 2014 4,576 5 View
In this paper, the author add a accesion no. for whole transcriptome sequence and I download it but .... i can't to open .sra file Pleas suggest me Thank a lot
08 August 2014 3,360 1 View
My tissue sample is a whole parasite (B.malayi) that stored in PBS at -80 C for 5 years. Can I extract RNA from the sample? Thankyou
11 November 2013 883 1 View
