Is there a good robust way to isolate plasmids from some Actinobacteria which potentially possess them? Is it a combination of enzymes/sonication or something else?
I isolated the plasmid from the strثptomyces in the alkaline method and using the lysozyme enzyme as per the paper (Birnboin HC and Doly J. A rapid alkaline extraction procedure for screening recombination plasmid DNA. Nucleic Acid Res.1979;7:1513-1523.)and the results were good.