What is the Timestep definition in the Conservation Element / Solution Element in 3D ?

15 November 2020 1,214 3 View

Help: How to install Skynet?

SkyNet is publicly available as an open-source software at https://bitbucket.org/jlippuner/skynet. However, i can not install it on my computer? Have anyone met the similar problem? First, CC=icc...

20 September 2020 6,912 16 View

Is free cytosolic GFP fluorescence excluded or reduced in the endomembrane system (ER, Golgi, lysosomes)? Why or why not?

As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...

08 March 2020 997 3 View

What are the techniques to validate qualitative research instruments?

I am currently doing a quasi-experimental research. I have decided to use both qualitative and quantitive methods in collecting the data. For the qualitative data, I have an observation card and...

08 October 2019 4,007 5 View

How many base pairs mismatches should i consider to design a specific primer? my templates have 1-2base pairs mismatch only when i aligned them?

i need to design a specific primer for each of my samples. but my samples, when i aligned them, they only have 1-2 bp mismatches, barely 3

17 September 2019 6,650 3 View

Can you recommend a fluorescent ELISA agent?

Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...

07 August 2019 2,604 5 View

What are the Arch Garch conditions?

20 April 2019 3,704 4 View

How a researcher can validate his upcoming research instruments?

I will be conducting an action research in one of the schools at my place of working and my supervisor has asked me to validate my instruments before I can use them during the research process. I...

11 December 2018 1,355 3 View

How to run longitudinal mediation with multiple (5 waves)?

Hi everyone! I'm doing a PhD in Clinical Psychology and I have some treatment data to analyse. The design is a 2 (condition: self-compassion, cognitive restructuring) x 5 (time: baseline,...

01 August 2018 4,502 3 View

Increased thermopower (Seebeck coefficient) with rising temperature? (p-type semiconductor). Is this the case for any p-type sc? What about n-type?

28 March 2018 4,475 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How long can synthesized peptides using SPPS be stored? Storage Conditions?

I have been synthesizing peptides on resin beads. I am wondering what best storage method is there to store the beads after synthesis before cleaving. And how long can they be stored in that...

02 February 2021 5,636 5 View