What is a best neuronal cell line?

Can we classify HV and LV lines based on line length?

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Issues with Permeabilization during Flow Cytometry?

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How to simultaneously perform multiple biochemical assays?

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Which cell line should we use to perform biological dosimetry experiments?

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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What could be some reasons why unstimulated monocytes are getting activated?

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Cássio Prinholato da Silva

Dear. Gülüzar,

There are SH-SY5Y;

PC-12 (neuronal like)

COS-8

Neuro-2a;

U-118 G

Depends on what you want to investigate!

Gülüzar Özbolat

Dear Cassio ,

Thanks in advance

One correction...COS-8 isn´t a neuronal cell, excuse me!

Caner Geyik

I am not sure if there is a `best` cell line. As Cassio said, it depends on what you want to investigate. SK-N-SH and SH-SY5Y are widely used. You can check their properties from ATCC website. They are neuroblastoma cell lines. You can further differentiate SK-N-SH by retinoic acid to express more glutamatergic receptors. Also PC12 cells can be differentiated to have neurites.

Alternatively, you can order neuronal stem cells and differentiate them to neurons before experiment (would be expensive though).

If you need particular characteristics or particular protein (for example you may want cells express X protein), you can always check Protein Atlas (Cell Atlas) database to see which neuronal cells express that protein.