phytochelatins is metal peptide bonds occur due to heavy metal stress, or it is a defence mechanism of plants. my queries is for best possible lab method to determine this pc bond in plant? i need with good reference protocol.
Please try electrophoretic profiling method for thiol-rich phytochelatins and metallothioneins. You can find the detail methods with Phytochem Anal. (2004) 15(3):175-83.