What happens if the media is double autoclaved immediately after the first autoclaving? I am going to inoculate the Trichoderma; will it affect the sporulation of the Trichoderma?
I am working on a project that involves female rats, and I would like to know how to manage the estrus cycle for the female rat model. Thank you in advance.
12 February 2024 4,524 2 View
we were all know for composting process, but due to fowl smell and other not happening problems, people avoid home composting which are required for save of environment? its a very simple and...
19 January 2024 1,474 3 View
My 3d building( 3 storey 6 bay 4 bay) showing very high stiffness and it is a combination of MRF and gravity frames. In place of gravity frames i want to release moment how can i achieve that.
09 January 2024 7,580 1 View
Hi, I'm curious about the exact organelle where ferroptosis takes place. Initially, I thought it was limited to the mitochondria and ER, but after reading a few papers, it seems it might occur in...
21 November 2023 2,194 2 View
If the compound is highly soluble in water and is recrystallised using water miscible solvents
30 September 2023 8,996 1 View
I have performed ab initio molecular dynamics simulations (AIMD) using Gaussian program. How to plot RMSD ( Root Mean Square Deviation) plot from MD.out file?
19 September 2023 5,961 0 View
I am currently pursuing my master's degree under the Department of Plant Pathology. I have keen interest to work on Trichoderma and its diversity and exploring up to the species level. But major...
11 July 2023 9,443 0 View
Hi, I have started working on whole-body plethysmography (SCireQ, iox by emka TECHNOLOGIES) of mice for my research project. Currently, I am recording the data and trying to figure out how to...
09 June 2023 7,950 0 View
Is it possible to spot AI/chatGPT-generated text?
04 April 2023 388 11 View
I have carried out XPS analysis of Ag based nanocomposite. XRD and TEM analysis confirms the presence of Ag. But it is not showing any peak related to Ag in XPS data. What might be the reason?
01 April 2023 3,241 5 View
Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container
30 July 2024 7,326 1 View
Thanks
22 July 2024 1,141 3 View
DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?
10 July 2024 7,526 3 View
Dear researchers, I've been working on aqueous plant extract sample, then I autoclaved them in 10 min to sterilize and added some preservatives. However, some of them showed bacterial and fungal...
11 June 2024 1,040 2 View
I'm currently studying the treatment of sewage wastewater and am interested in understanding the impact of autoclaving on the concentration of heavy metals. Specifically, I would like to know if...
10 June 2024 2,579 4 View
The buffer wasn't filtered.. Could this be the cause? Can we autoclave phosphate buffer to resolve this issue?
10 June 2024 3,969 2 View
I am using CTAB method (Naser M. Salameh et al., 2014) for fungal DNA isolation and purification. During reagents preparation I am not sure weather I should autoclave 2X CTAB and Wash buffer. So...
09 June 2024 8,470 2 View
I used Vero E6 cells and Mayaro virus. The virus was diluted in DMEM without FBS. The plaque media was 2X MEM, prepared with sodium bicarbonate and L-glutamine, adjusted to a pH of 4 with sodium...
03 June 2024 519 0 View
the autoclave has been smelly and seem likely yellow after my synthesis and this changes may effected on other synthesis, so I want to clean PTFE from all sulfur contamination that remain on...
31 May 2024 2,985 0 View
I am trying to get a new Biobase BKQ-B120L autoclave to work however it does not go through its cycles. It will start up and go to about 110C and then remains there untill the ERR alarm sounds....
21 May 2024 4,885 1 View