How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

CFD steady state simulation not converging even after large number of iterations for multiphase flow?

Dear All, I have been working on multiphase flow through a complex geometry having porosity 0.25. I have patched it with oil and high-salinity brine. At the inlet I am flowing low-salinity brine....

04 December 2020 4,506 3 View

Absolute values in Leptin ELISA kit are higher than normal. Does it depend on washing procedure?

I have been using mouse leptin ELISA kit (Catalog number (90030) from crystal chem. I have been doing manual washing between my steps as I didn't have automatic washer instrument. Does this cause...

12 August 2020 4,352 4 View

What are the best tools and study protocol to study the beneficial soil microorganism and the symbiotic agents?

I am going to conduct the field survey to collect the major soil living organisms, beneficial insects, natural enemies, and decomposers and symbiotic agents. For each study, I am looking for...

28 July 2020 8,298 3 View

Which book/study material would be best for a beginner in optimization research field ?

power system optimization problems and optimized unit commitment

20 April 2020 6,248 12 View

How to generate a magnetic field around a sand packed column?

Hi all! I want to make a lab scale setup. I want to generate a magnetic field around a sand packed column. I want to inject magnetic nanoparticles in th sand column and want to track them...

07 April 2020 1,393 1 View

What is the best way to euthanize invertebrate insects in the field for RNA sequencing?

Field work will be conducted in very harsh, windy, and cold environment.

08 March 2020 8,387 3 View

What could be the best way of staining our DNA during gel electrophoresis using Gel Red? Post staining or pre-staining and what concentration?

We use gel red in our lab to stain our DNA for gel electrophoresis. The bands obtained are wavy and smeary sometimes. How shall we overcome this problem?

20 February 2020 3,302 3 View

Is it possible to remotely detecting location of fluorescent nanoparticles in a core sample using a quencher?

Dear all, I have injected fluorescent-based nanoparticle fluids in core samples. Is it possible to remotely detect the location of fluorescent nanoparticles in a core sample using a quencher? Thanks

11 January 2020 3,102 3 View

Should oil recovered after water flooding be similar for all core flooding experiments to compare oil recovered by different surfactants in CEOR?

I am performing core flooding experiments to compare percentage of additional oil recovered during surfactant flooding in a sand pack column. Is it necessary that after secondary waterflooding...

16 December 2019 2,886 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

What makes yellow color appear in phosphorus sample after autoclave digestion?

I was using autoclave for digesting water samples for Total phosphorus measurement with ascorbic acid method. The autoclave was set to perform 2 hours digestion and around 121 Celsius degrees. I...

25 January 2021 206 3 View

Disposing Congo Red Agar?

Hi all, Hope you are well. My lab is planning to use some Congo red agar (0.01%) for Shigella flexneri, and we are wondering how do you dispose of the used agar plates. The Shigella contaminated...

13 January 2021 9,823 2 View

How can Sea water impact the hydrodynamic size of Nanoparticles differently than the same Sea water if it is autoclaved?

I am using the same sea water as autoclaved as well as non-autoclaved after adding the nanoparticle. I am getting different Hydrodynamic size for the same nanoparticle.

06 January 2021 3,184 3 View

Please share some research papers on the synthesis of Mn3O4 nanoparticles via Hydrothermal method by using Manganese(II) nitrate hydrate?

05 January 2021 9,040 4 View

How to get dry petri dishes in glass after wet sterilization?

Hi, I warping my plates with paper, later put them in the autoclave (use wet sterilization cycle) but still some plates are get wet after ends the cycle. How to get dry the petri dishes? and if...

13 December 2020 8,062 4 View