Format Mothur database to run through dada2?

I am looking to run illumina miseq data through dada2, but the limited identification of this gene means there are no pre-build databases. I have a database built in MOTHUR format, does anyone...

30 November 2020 8,228 3 View

How do you co-culture astrocytes and neurons?

I am planning to co-culture primary astrocytes with immortalized neurons GT1-7 (both from mice). What is the best way to do this?

05 November 2020 5,040 4 View

Has anyone used paraffin oil (liquid paraffin) instead of saturated alkane mixture in determining RI?

I would like to know if anyone has tried, or has any experience, in analyzing liquid paraffin with GC/MS and recording retention times. As hypothesized, this alkane mixture could maybe serve as a...

14 November 2019 8,547 3 View

Can I construct and ARDL model and test for cointegration with i(1), i(0) and i(2) variables?

Hi, I am currently doing a dissertation on demographic determinants of household savings in China during the One Child Policy, using time series. My variables are as follows: Household Savings- %...

21 July 2019 9,085 21 View

Rotating airfoil simulation - Fluent ansys - CL, CD?

Hello guys, I am in real need, I am working on my diploma thesis and the deadline is really close. I am having a bit of trouble with getting correct results of CL and CD from simulation over a...

13 April 2019 6,666 7 View

How do I increase the quality of the resulted video from Ansys Fluent simulation?

Hey! I am working on a flow analysis over a 2D airfoil.. I simulated the situation at different angles of attack, put it at 400 interations and everything works well in programme, although after...

09 April 2019 8,878 3 View

Is it possible to extract lift and drag curve from Ansys fluent?

Hey! I am currently working on flow analysis over 2D airfoil in Fluent ansys... I am new in this programme so after a day of hard work I managed to make the simulation according to the youtube...

09 April 2019 519 3 View

What cell culture contaminant turns media yellow but not turbid?

20 May 2015 4,389 11 View

Have anyone experienced that A549 and U251 cells grow in regular wavy lines in T75 flasks?

I have occasionally experienced that the two cell lines have grown in very regular wavy lines from the stopper to the opposite end of the flask. The cells seems to be okay except that they won't...

15 April 2015 9,034 2 View

Courses in Bioinformatics and NGS

Can anybody recommend specific courses in Bioinformatics and Next Generation Sequencing/ Deep Sequencing? What intense / short-term courses are available out there, and what are your experiences...

26 August 2013 9,904 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View