Dinum Herath

7 Questions 32 Answers 0 Followers

Questions related from Dinum Herath

Finding of allele differences in polyploidy spp?

I am wondering anybody has a good suggestion to find the allelic difference in a particular gene in tetraploid spp. I tried the Sanger sequencing method. But it looks so hard to amplify all four...

23 February 2021 2,130 7 View

Is any one have a suggestion to improve leaf expansion in tissue culture?

Some of my stock plants leaves are not expanded well. I tried with different auxins. but it does not work. Does anyone have a different thought about new hormones?

10 June 2020 1,365 15 View

What is the important of checking OD value in Agrobacterium transformation?

Are we really monitoring the concentration of the bacteria or bacterial growth.

26 May 2020 5,692 4 View

What is the possible relative expression value for 35S: over expressed gene in transgenic plant?

I was just checking the expression of my candidate gene in the transgenic plant. when I was looking at the relative expression of this gene in an overexpressed transgenic plant (35S/OX plant) it...

23 May 2020 6,512 0 View

What is the best method to keep tissue culture plants survive in lockdown period?

I am wondering about any good practices to maintain plant cultures in long-duration without subculturing. because we are experiencing a long lockdown period without entering to the lab.

09 April 2020 4,915 32 View

Any body have an idea about use of Hygromycin in plant transformation experiments. Which concentration is suitable to select the transformed calli?

Hygromycin is try to use for selection over the Kanamycin.

21 October 2018 2,030 0 View

How to write the concentration of the media by mg/L or mg/l?

I found both forms in research articles. what is the correct way of writing?

03 October 2017 8,601 3 View