So, I am trying to purify the protein from the pMal amylose resin column system. However, i have a hard time after the cleavage by factor xa. my protein of interest precipitate out... I tried to switch the column buffer to pbs +10% glycerol. However, it is still not working. I am trying to purify the protein and send to the antibody company. I understand that I can try the his tag Protein and send the MBP+Protein of interest to the company. Do you guys by any chance to suggest some PBS included buffers or other solutions? Thx a lot.