Does anyone know have to detect intracellular labile iron pool in live cells? Or does anyone have recommendations of essay kits for such detecting purposes?
Dear Zuo Xiang
One of the alternative methods to using an iron chelator for quantification of the intracellular iron content of cells will be using QuantiChromTM Iron Assay Kit (Bioassay Systems, DIFE-250) which involves preparing a cell lysate and measuring the Optical density of the sample at 590 nm ( ICP-MS).
The cellular labile iron pool (LIP) is a pool of charitable and redox-active iron, which is transitory and serves as a crossroad of cellular iron metabolism. The chemical identity of this pool has remained poorly characterized due to the multiplicity of iron ligands present in cells.
Kindly check this paper:
Article Molecular Imaging of Labile Iron(II) Pools in Living Cells w...
Masoum Mohammadi Gharagoz Thank you so much for your generous time. I've checked the assay kit that you recommended, while regretfully I founded it could not discriminate from intracellular Fe(II) and Fe(III) and hard to visualize subsellular localization, I'll be keeping looking for it. Again, thank you so much for your answer.
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