Hi Everyone,
I am trying to get mice skin paraffin sections after 4% PFA fixation. However, after tissue processing, the skin become a little bit harder. And When I cut the paraffin sample, it always easily broken and get stuck when I cut the skin. It seems a little bit tough to cut the skin sample. The skin in the paraffin stock looks white like the picture shows( maybe the paraffin get harder in skin? ) Sometimes when I melt the skin and reembed it, the cutting becomes much easier.
I have never done paraffin section before and I really want to know how to get good skin paraffin sections.
Does anyone have any experience doing paraffin embedding of mice skin and any advice about paraffin section.
Thank you a lot!
https://www.tse-systems.com/service/indirect-calorimetry/?utm_term=mice&utm_campaign=Product+Campaign+-+PhenoMaster+-+World+-+EN&utm_source=adwords&utm_medium=ppc&hsa_acc=6040599756&hsa_cam=13137842121&hsa_grp=130978366788&hsa_ad=522144455172&hsa_src=s&hsa_tgt=kwd-10032756&hsa_kw=mice&hsa_mt=b&hsa_net=adwords&hsa_ver=3&gclid=CjwKCAjwqZSlBhBwEiwAfoZUIFt_oYV1FeIHF892NDlXJMS_ix8f99n8p3OUbbiWkACD2oscZczb_xoCGrEQAvD_BwE
Hi, I don't know if this is helpful, but I always orient the skin vertically when cutting. From the marks on your block I think you orient it horizontally?
As I said, I do not know if this helps, but maybe it's worth a try :)
Doubt, is it paraplas or pure paraffin? and the second is, have you already tried it with 10% Buffered Formol?