What happens when you use different primers to amplify the same region of DNA, and you get different information?

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Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

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How to compute the distance in km between two points inside the Earth?

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Is there any databases of sequencing data for AD mouse models?

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Does anyone have problems performing CRISPR KO in human primary T cells?

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How do you transmit and evaluate ethics and moral values in you teaching activity?

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What are the applications of remote sensing and GIS in transportation, what are the types of sensors you will require?

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Having issues understanding the particles/mL to molarity conversion, I've seen many different way but non of them seem to match one an the other?

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How to migrate a graph database to SQL?

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Can anyone explain the calculation process of integrated biomarker response (IBR)? Any specifical software?

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In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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How to calculate the annealing temperature of a primer pair?

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Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

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What is the ideal efficiency for a qPCR?

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MiRNA qpcr problem?

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Has anyone used the Illumina ITS1 primer sets?

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QPCR reaction efficiency with odd results what does this indicate?

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Abhijeet Singh

I don't understand your question. What do you actually want to ask?

What do you mean by information? What have you done for experiment?

Katie A S Burnette

Different pairs of primers can amplify a different sized product from the same template DNA.

I can also tell you that COI and other "universal" or "barcoding" primers will often amplify DNA from an unintended source (e.g. primers for 16S designed for fish will easily amplify human DNA).