Has anyone been successful in seeding microglia from adult mice?

Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...

14 October 2020 4,572 3 View

Can I use the cryostat to slice brains that were perfused with 4% PAF and Gadoteridol?

Hi everyone Im trying to slice my mice brains with the cryostat, however, I haven't been successful, the tissue seems to stretch and then it breaks. Let me tell you the story about these brains:...

25 August 2020 6,479 4 View

What is the difference between KPIs and Balanced Scorecard ?

Measuring business performance Organisations face the intense pressure of carrying out their processes efficiently, effectively and sustainably every day (Ghosh & Fedorowicz, 2008). Companies...

30 March 2020 1,553 4 View

Ghost signals in LC/MS scan mode. Contamination?

Dear experts across the world, today I am looking for advice on a new field my lab is starting to work on: LC/MS. We are using an Agilent 1260 Infinity system, with a 6120 Quadrupole LC/MS. We do...

17 December 2018 6,718 3 View

Is there anyone that has used Electron Microscopy Sciences grids for EV analysis?

Hi everybody. I wonder if there is someone here that has successfully worked with TEM grids sold by Electron Microscopy Sciences for the analysis of extracellular vesicles (microvesicles and...

13 November 2018 9,993 1 View

How to compare 2 variables where 1 has 2 possible values and the other one are experimental data?

I made 30 assays testing the capacity of 2 chemical products in the removal percentage of dirt. 15 assays were made with the chemical product `0` and 15 with the chemical product 1. I obtained a...

23 November 2017 8,831 1 View

How to compare a 3 sets of data in order to sort out how 2 of these data influence the third one?

Hi, I have a 3 set data like the image There is a tool to say what is the most important variable in the removal, pH or dosage? I was thinking in a PCA (principal component analysis) however I'm...

10 November 2017 6,864 7 View

How to compare 2 variables where 1 has 2 possible values and the other one are experimental data?

I made 30 assays testing the capacity of 2 chemical products in the removal percentage of dirt. 15 assays were made with the chemical product `0` and 15 with the chemical product 1. I obtained a...

10 November 2017 8,746 1 View

How well does SEM (Structural equation modeling) fit with mixed methods studies?

Dear colleagues: For decades, certain qualitative techniques such as interpretive thematic analysis have worked in synergy with quantitative techniques such as bivariate analysis and multiple...

09 October 2017 8,448 9 View

How well does SEM (Structural equation modeling) fit with mixed methods studies?

Dear colleagues: For decades, certain qualitative techniques such as interpretive thematic analysis have worked in synergy with quantitative techniques such as bivariate analysis and multiple...

02 October 2017 3,450 9 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View