I have isolated fungi from plant but not able to identify on microscopic level
this is some Aspergillus sp.
send some pictures of the colony of the culture
Dear , Vipin Nagda
It is one of species of Aspergillus spp. but you need some characters to know the species , I shall send them to you later
Best wishes
Dear, Vipin Nagda
Some characters are needed to know the species of Aspergillus
1 - Macroscopic characters such as : color of colony , the margin of colony , the texture , the type of media and the growth rate .
2 - Microscopic characters such as :
a - Phialides , predominantly uniseriate or predominantly biseriate.
b - the vesicle shape
c - the conidia size and shape
d - The surface of conidia roughed or smooth
e - Conidial head radiate or loosely columnar
MY REGARDS
Hi!
It seems to be an Aspergillus but you must to look for species identification as Yehia told you. Colony morphology depends on culture media...useful for identification, also (i don't know what culture media you have used).
I find this articles usefuls:
https://file.scirp.org/pdf/AiM_2015040114024967.pdf
https://pdfs.semanticscholar.org/41f0/2b3f74092d7adb6c9165669a3a13b6382a1e.pdf
Best wishes!
Beatrice
Hi , Vipin Nagda
You can also depend on the reference :
((A laboratory guide to the common Aspergillus species and their teleomorphs)) By : Klich and Pitt , 1988. It, s good guide.
With my best wishes
Hi Vipin Nagda
You must prepare many slides from young cultures 3 - 4 days old in order to see most characters of the fungus for easy identification .
Good luck
Dear Vipin
Although the pictures are not sharp enough, the fungus could be a species of Aspergillus.
Aspergillus it is. Recently my students isolated quite a number of this fungi for their final thesis
It is Aspergillus species. If image from the plate was available, identification of species was easier,
It looks like Aspergillus.You can upload the wet mount preparations of culture in any stain such as Lactophenol Cotton Blue stain or Narayan stain or PHOL stain with higher magnification so that morphology can be easy to study.
The organism is an Aspergillus species, do further studies to confirm its species level.
Aspergillus . The morphology is uncommon. Study further it is interesting.
It looks like Aspergillus, but it is quit necessary to show the morphology of fungal colony together with mycelium and conidiophores
Good luck
This is Aspergillus. Probably uniseriate sterigmata group. Note the colony character on Czapek agar, study columella, sterigmata, spore and conidiophore. Consult keys to identification of Aspergillus. Consult an expert on Aspergillus. They give us correct answer known Aspergillus or new one. Good luck.
The photograph of fungus isolated from plant gives a similarity to Aspergillus.You should prepare more wet mount preparations either in lactophenol cotton blue stain or PHOL stain or Narayan stain and try to study the morphology by taking help of some Medical Mycology books or an expert. The later two stains,namely PHOL stain and Narayan stain are developed by us.You can download both the publication from Research Gate or Academia.
Wish you best of luck in your scientific career.
That is a species of Aspergillus .For more information about the species you can culture the mold on Malt Extract Agar at 25 oC for morphology characteristics.( like the size. of colony ) .Also use different Czapeck agar as the reference (identification of common Aspergillus species by Maren A. Klich ).
If that is a new one Aspergillus you can use Molecular methods and send the DNA for sequencing .
It is certainly Aspergillus sp you have to use conidial colour, phialides'' organization, conidial morphology, surface characteristics of the colony and reverse colour of the plate, morphometric characteristics , and the characters as seen in oar meal agar and other media, and you have to consult 'The Genus Aspergillus'- a manual by Thom and Rapper. for species identification.
Hi, examine the culture when young about 3 days. Examine from culture margin while the hyphae and conidiophores will appear well if it is Aspergillus.
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