How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Can there be a decrease in the membrane time constant and an increase in membrane excitability?

In my data set, I am seeing exposure to my peptide of interest leads to significant increases in action potential firing responses. But in the same cell types, I am seeing a decrease in the...

17 November 2020 5,441 4 View

What is the best anatomic plane to study BNST neurons using ex vivo slice culture in a rat?

I am looking to study neurons of the bed nucleus of the stria terminalis. I have seen reports using coronal sections. Others present the distinct nuclei in transverse sections. What is the best...

22 October 2020 3,343 1 View

Can anyone recommend a protocol for prolonged storage of mouse brain tissue for eventual paraffin embedding?

My lab is looking to perform IHC staining on the brains of several dozen mice, and we have a need to store the perfused brains for up to a month before we have access to paraffin embedding. Can...

14 October 2020 6,256 4 View

Does anyone have a good protocol for taking soil samples and extracting fungal DNA?

I'm trying to compare fungal communities in the soil of two neighboring ecosystems. I need a protocol for soil sampling to later use for sequencing fungal communities.

25 August 2020 6,830 3 View

Is there a minimum optimal diameter for neural stimulating electrodes?

With the 1.27mm diameter of DBS electrodes often leading to the development of scar tissue, and evidence that electrodes with diameters near the size of neurons, approximately 30 microns, are less...

26 March 2020 1,782 3 View

Why this orange hue on my gel electrophoresis?

A new batch of 1x TBE buffer was prepared and I was testing it out (result is attached). I am wondering why half the gel is lighting up orange, as I have never seen this before. I have not changed...

12 August 2019 5,209 4 View

How can I resolve problems of maintaining tissue integrity during immunofluorescence for paraffin-embedded sections?

18 October 2018 3,208 4 View

How do I determine a proper loading control for my western blot assay using whole tissue homogenates?

I am running a western blot assay using whole tissue homogenates. The homogenates are from collected mouse brains and I am using two groups an Ames dwarf mouse and a wild-type mouse. I see that...

14 October 2018 3,121 2 View

What is the strength of signal from ROSA mTmG mice in fixed, paraffin-embedded tissue?

I am looking to use ROSA mTmG mice with a cell-specific Cre. I will have tissues fixed in 4% PFA, paraffin-embedded, and sectioned for studies. Is the signal from tdTomato and GFP strong enough...

12 April 2018 2,826 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View