What factors of/in the structure (computational model) should I consider for proper protein expression?

Venkata Vamsi Bharadwaj Yallapragada @Venkata-Vamsi-Bharadwaj-Yallapragada

13 March 2017 0 7K Report

I am trying to express a fusion protein and I have started with the computational model for the protein construct. I have made different arrangement fashion combinations of the secretion signals, actual protein of interest, tags etc.

I am now looking at the structures using rasmol, as far as I know, I am trying to look if my

1. secretion signal is well exposed,

2. the paratopes are well exposed

are there any other factors that could influence the expression that I can see in these structure models?

anything that I have to consider or any suggestions?

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