Venkata Vamsi Bharadwaj Yallapragada

6 Questions 2 Answers 0 Followers

Questions related from Venkata Vamsi Bharadwaj Yallapragada

Why a colorimetric assay is usually not in green?

Various colorimetric assays we use are blue/purple/pink/red but not in green shades. Is there any particular reason for this?

31 May 2020 6,267 1 View

What are some physical methods to determine binding affinity of a protein?

What are various options available while determining the binding affinity of a protein to it's target? In my case the target is also a protein. Are there any standard physical methods to...

10 March 2020 8,441 8 View

How many stop codons to use, for expressing a protein in a mammalian system?

A few of my co-researchers have mentioned that it is good to have more than one stop codon at the end of the protein, just to make sure that translation stops. Now, do we need more than 1? One of...

11 May 2017 4,949 4 View

What factors of/in the structure (computational model) should I consider for proper protein expression?

I am trying to express a fusion protein and I have started with the computational model for the protein construct. I have made different arrangement fashion combinations of the secretion signals,...

13 March 2017 6,752 0 View

What are some good plasmids to start with?

I am trying to express an antibody fragment first in bacterial and in mammalian cells later, what are the best available options for each of them, to start with? Any suggestions from your previous...

13 March 2017 3,997 1 View

What will happen if I stimulate my cells with a ligand for 8 hours?

I am working on a project where I have to extract mRNA from a cell line. Usually for mRNA, we stimulate for 2-4hours (or even lesser), but what happens if I stimulate for 8hours or more?Would it...

27 June 2016 6,633 2 View