Remove chlorophyll prior to extraction using activated charcoal.

i agreed with Ibrahim and Alok sir's answer but Jecinta if you give some more information then its easy to understand because as per second scientist view you have to give charcoal treatment but its also possible that charcoal also removed your interested molecule.

Regards

I am using plant leaves (plant material). I had negative results with activated charcoal as other precious compounds were also adsorbed.

I think you not read my answer in my answer i write this thing !

You can use column choreography and its a best method for that, and if have no problem then share some more information about your targeted molecule so according to that i try to make protocol for that.

If you have contaminated extract with you don't discard it. Just do column chromatography of the extract with chloroform:methanol:toluene (8:2:1) as eluting solvent. Make the column using silica gel for column chromatography (80-120 mesh) and pack it using wet packing technique using chloroform.

When you will run your contaminated extract in the column, the very first eluate you will get will be chlorophyll. Perform a TLC of the eluate in the same solvent system to find a orange band at the solvent front in UV 366. Go on doing this till the eluate is free of chlorophyll.

This way you can save your so called contaminated extract too.

For obtaining chlorophyll free extracts of leaves try hot water 60-80 degree as extractant. This aqueous extract was then partitioned with hexane and chloroform which will totally remove chlorophylls from the extract.

Extract your plant material in hexane in Soxhlet for removal of chlorophyll.

Use A27MP or equal resin with water extract

I have a question regarding the extraction methods for chlorophyll without the use of solvents of any kind. Here is an example of a companies description of their extraction process in the post below. Any insights as to what they may be doing would be greatly appreciated.

PURE CHLOROPHYLL.

NOT CHLOROPHYLLIN.

WE DON'T USE SOLVENT/WATER when extracting Natural Chlorophyll from Mulberry leaves.

PLANT LIQUID EXTRACT fromMULBERRY LEAVES containingActivated Natural Chlorophyll

MANUFACTURER DIRECTLY SELLS - CHLOROPHYLL A CONTENT is remarkably high as mush as 559.2mg PER 100G MULBERRY EXTRACT.

Chlorophyll A and Chlorophyll B are entirely activated in our natural chlorophyll.

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[Our 'CHLO-ORE' containing Activated Natural Chlorophyll] : We extract natural chlorophyl as it exists in the nature with TPLEC (The technology to extract natural chlorophyl physically in bulk) developed by us without destruction of nutrients like Mg, Phythol and Carotinoid. We don't use any solvent, Cu, Fe, Co to extract chlorophyl. The body can absorb this easily. No destruction of nutrients. No side effect.

Actually it's very difficult to produce Chlorophyll as the way of existing production and nobody can produce big volume of real Chlorophyll in the world so far due to extremely high production cost.