Which is the best the detector in HPLC for test cananbinoids?

In the HPLC, we can use differents detector methods to quantify the cannabinoid content from the flowers of Cannabis sativa. Most populars detectors are: 1. UV 2. Diodo Array Detector 3. Mass...

19 September 2019 459 3 View

BD CBA cytokine kit assay use with BDFACSDiva and Flowjo?

Does anyone know if the CBA cytokine assay offered by BDbiosciences is compatible to run with BDFACSDiva software on a flow cytometer (vs the BDFACSSuite software that is recommended in the user...

15 January 2019 5,663 5 View

What is the best way to convert Multiband sequence DICOMs into Nifti?

Normally for functional images in the past I've used Xiangrui Li's Matlab script or mricron's dcm2nii but this is the first time I am working with Multiband data and have been running into...

12 April 2018 5,553 3 View

When performing multicolor flow cytometry can one use several different color panels in one experiment?

So let's say I have 3 samples and I am interested in testing different fluorophore-conjugated antibodies against certain antigens by using 12 different colors. Howeveer, aach sample only has 10...

05 April 2018 4,083 2 View

Why do I get fluctuating peak areas?

I carried out an experiment aimed at confirming the presence of aspirin and to quantify the abundance of asprin using standard addition. And using HPLC instrumentation. From the result, the...

05 April 2018 5,916 6 View

ELISA assays: Negative results for known positives?

I am testing insoluble homogenates diluted to 0.25M guanidine HCL. Standards for every kit tested work beautifully (standards also in guanidine HCL dilution. The samples are from human gene KI...

22 January 2018 8,822 2 View

Is there a formula in computing the retention factor values of lichen substances for the solvent dichloromethane?

I am currently working on the identification of lichen substances (secondary metabolites) of manglicolous lichens and I only used thin layer chromatography. The solvent I used is dichloromethane....

06 October 2017 7,939 1 View

When staining targets from 2 different species for flow cytometry, can you use 2 different primary Abs against different species in the same sample?

(if you staining for a mouse target and human target within one mouse (ex, chimera), can this be stained in the same tissue sample? Or should samples be stained separately?)

09 August 2017 7,149 2 View

Can anyone explain why I get a lower effect estimate using two-stage logistic analyses compared to straightforward logistic regression?

I am using a two-stage approach (whereby admissions to hospital are nested within patients) to account for multiple admissions of individual patients during a study period. Intuitively it makes...

21 September 2016 8,379 4 View

Are there available ID keys to marine epiphytes associated with seagrasses?

16 August 2016 395 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View