I have a list of alternatively expressed exons and they are described as "E5-1" for example. I understand the E5 means Exon 5 of the gene, but what is the -1 for?
What is the source of the annotation ? Could it be related to alternate splicing or perhaps to tissue specific mRNA ?
I am working with about 400 tumour samples looking for alternative splicing in a specific gene using RNAseq. Should I be aligning the RNAseq libraries to each patient genome (so download 400...
05 June 2019 3,206 5 View
I downloaded the clinical data from the original TCGA article from 2012 (https://www.nature.com/articles/nature11412#supplementary-information). I am interested in studying patients that present...
04 May 2018 9,128 0 View
I'm going to be working with Exon array data and was wondering what free programs are good and available to use?
01 February 2016 4,132 3 View
I'm using Invitrogen superscript II and SsoAdvanced SYBR green for qPCR (pcrs work but I'm not sure if these systems are completely compatible). Annealing is at 63 and shows no primer dimers at...
05 June 2015 6,968 5 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View