Sean Seltzer

5 Questions 28 Answers 0 Followers

Questions related from Sean Seltzer

Should I align tumour RNAseq libraries to reference genome (hg19) or each patients genome?

I am working with about 400 tumour samples looking for alternative splicing in a specific gene using RNAseq. Should I be aligning the RNAseq libraries to each patient genome (so download 400...

06 June 2019 3,163 5 View

Are the TNM data in the TCGA BRCA files about current patient status or status at diagnosis?

I downloaded the clinical data from the original TCGA article from 2012 (https://www.nature.com/articles/nature11412#supplementary-information). I am interested in studying patients that present...

05 May 2018 9,090 0 View

What does this Exon annotation mean?

I have a list of alternatively expressed exons and they are described as "E5-1" for example. I understand the E5 means Exon 5 of the gene, but what is the -1 for?

04 April 2016 5,622 1 View

What programs are good to use for Exon Array data or other microarray data?

I'm going to be working with Exon array data and was wondering what free programs are good and available to use?

02 February 2016 4,082 3 View

Can anyon explain why these melt curves look this way between 70 and 80 degrees?

I'm using Invitrogen superscript II and SsoAdvanced SYBR green for qPCR (pcrs work but I'm not sure if these systems are completely compatible). Annealing is at 63 and shows no primer dimers at...

06 June 2015 6,926 5 View