What does "enriched DNA" mean?

Maria Giovanna Lupo @Maria_Giovanna_Lupo

05 May 2015 0 2K Report

In many papers regarding sequencing techniques you can read that the DNA samples were enriched ... but what does "enrich a DNA sample" mean?

Badges
Science topic

More Maria Giovanna Lupo's questions See All

How can I retrieve a weighted matrix for a DNA Bindin Site motif (Smad binding site in my specific case)?

I Know that the consensus sequence for Smads is the so-called CAGA-box, that comprises the main CAGA motif, (i.e. CAGACA),but also the CAGA-like motifs. I'm interested in retrieving a weighted...

05 June 2016 7,671 2 View

Can anyone suggest me if there are online bioiformatics tools that can help me in finding short motifs in a protein sequence?

I have to know if in my protein there is a specific short motif (3aa), how many times it occurs and in which positions. Does anyone know if it exists any online tool that allow me to perform this...

05 June 2016 3,745 4 View

Which are the genetic causes of Hypertension?

We all know that hypertension is mainly due to our bad habits like eating too fat food and doing very little sport. Moreover nowadays our very stressing days don't help us and our blood pressure....

04 May 2014 9,228 6 View

Are there in-depth studies on non-genetic heterogeneity in eukaryotes?

I'm very interested in understanding what non-genetic heterogeneity is and what the main causes that trigger it are. I know there are a lot of studies on this phenomenon in bacterial clonal...

03 April 2014 2,411 6 View

Does anyone know reviews on the various genome Sequencing methods?

Does anyone know reviews on the Genome Sequencing that explain in some detail the various sequencing methods, starting from those of Sanger and Maxam-Gilbert up to those of the Next Generation...

02 March 2014 9,595 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View