In many papers regarding sequencing techniques you can read that the DNA samples were enriched ... but what does "enrich a DNA sample" mean?
I Know that the consensus sequence for Smads is the so-called CAGA-box, that comprises the main CAGA motif, (i.e. CAGACA),but also the CAGA-like motifs. I'm interested in retrieving a weighted...
05 June 2016 7,717 2 View
I have to know if in my protein there is a specific short motif (3aa), how many times it occurs and in which positions. Does anyone know if it exists any online tool that allow me to perform this...
05 June 2016 3,789 4 View
We all know that hypertension is mainly due to our bad habits like eating too fat food and doing very little sport. Moreover nowadays our very stressing days don't help us and our blood pressure....
04 May 2014 9,271 6 View
I'm very interested in understanding what non-genetic heterogeneity is and what the main causes that trigger it are. I know there are a lot of studies on this phenomenon in bacterial clonal...
03 April 2014 2,475 6 View
Does anyone know reviews on the Genome Sequencing that explain in some detail the various sequencing methods, starting from those of Sanger and Maxam-Gilbert up to those of the Next Generation...
02 March 2014 9,632 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View