Isn't it the "amplification and quantification of the desired gene fragment or is something else ????
Maybe you should define a little better the context... but the principle of PCR is basically the amplification of a target use by mean of two primers designed at the extremes of the target sequence and with opposite orientation. In the "concept" is also included the idea of using different temperatures to optimize DNA denaturation, primers annealing and primer elongation using a DNA polymerase. But the quantification itself is not in the original "concept" i think, because there are different methods to quantify the expanded DNA and it is possible to do other with DNA other than just amplificaiton.
Yes, I think you are right. The "principle of PCR" is to amplify a copy of DNA fragment into many many copies of them.
**Definition of 'Principle': a basic truth or theory; an idea that forms the basis of something (Merriam-Webster Dictionary).
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