Have been using the Nielsen method of degree of hydrolysis however I obtained a negative value does this indicate the protein contents is too low in samples
Dear Researchers, My research team is conducting a eating disorder related research in Malaysia and we would like to use the Malay translated version of EAT26/40. I have tried to email the...
24 January 2021 8,925 2 View
Base on my study, there is about 85 kw of heat will generate from a combustion of methane gas. I want to cool down the hot air by passing it through a heat exchanger. According to my previous...
15 November 2020 167 3 View
Is there any other new technologies or new methods to extract IPA from wastewater instead of distillation and salting out method?
20 August 2020 9,721 2 View
1. May i know the values for the concrete damaged plasticity in abaqus? it is default value or depends on the concrete properties? btw, where to obtain the values? 2. In the real case, there are...
27 February 2018 6,650 6 View
Dear all, I am currently trying to do a 3-way Gibson Assembly using 3 DNA fragments of size, 2.4 kb, 4 kb and 6.3 kb. The final ligated product will have a size of 12 kb. My protocol involves...
08 January 2018 4,181 5 View
Dear all, I am working on a synthetic biology project and will be using a titratable expression system (pLac). Since this system gives leaky expression, I would like to introduce a lacI repressor...
11 January 2016 3,267 9 View
Hello. I am using a CL-1000 UV crosslinker from UVP to irradiate my reagents for Multiple Displacement Amplification before using them for Whole Genome Amplification. According to a reference, it...
16 August 2015 1,861 2 View
Dear all, I am currently working on a synthetic biology project where I have to construct my own plasmid for protein expression. In regards with protein expression, I have chosen the inducible T7...
05 February 2015 6,904 3 View
Dear all, I am now working with a bacterial gene cluster and located 26 internal promoters within this cluster. Most of these promoters are found on coding region and I plan to remove them for...
27 October 2014 430 8 View
As the penetration of grid-connected solar energy increases, is it technically possible to replace most of the conventional power generations including coal, gas and natural gas in modern power...
01 January 1970 3,320 2 View
I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...
14 February 2021 6,049 3 View
Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...
27 January 2021 7,924 1 View
I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...
20 January 2021 7,874 4 View
After acid hydrolysis and incubation in the dark using Taka-diastase enzyme, 30% sulphuric acid was added to stop the enzymatic reaction. The solution was diluted to the mark and some of the...
16 January 2021 3,740 4 View
Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...
13 January 2021 9,449 0 View
I dont have access to the needed enzymes for CF hydrolysis, so I'm wondering whether I can do the hydrolysis solely with the use of acids? Has it ever been done before?
08 January 2021 1,243 3 View
Hi, I've determined the glucose content in Cellic CTec2 enzyme blend and it is 12-14 mg per 100 mg of enzyme blend. Could anyone share with me your results so I can compare it with mine?
08 December 2020 6,312 3 View
Hello everyone, may I ask about the purpose of adding ultrapure water after incubation for 30 minutes at 80 degree Celsius (under saponification process of vitamin D? Secondly, what is the...
02 December 2020 8,533 4 View
We do use enzymatic hydrolysis in the extraction of abused drugs in blood and urine. For example, if I am using 150 kU/ml b-glucuronidase and the sample volume is 100 ul, what is the optimal...
16 November 2020 2,930 3 View
I am making Poly Glycerol Sebacate nanoparticles by solvent evaporation and nanoprecipitation, but material got hydrolyzed upon adding water. I want to replace aqueous phase. Which chemical should...
21 October 2020 8,319 9 View