To understand how glucose is being metabolized in our cell conditions, 13C Glucose was used as a tracer. To determine the carbon contribution from tracer glucose in each metabolite, the MPE was calculated. However, some MPEs came out as negative. I am relatively new to metabolomics so I am hoping someone can help explain what a negative MPE means in terms of glucose carbon contribution to these metabolites. Or if you can point me to literature that provide an explanation and examples, if will be greatly appreciated.
Thank you
Isotopic ratio work can be a bit tricky. It is better to use the isotopic vector than just the isotopic ratio of the two isotopic peaks. The math is a bit more complex, but still can be done in a spreadsheet. You get much more reliable results. See link below:
Method Isotopic Vector Angle Analysis in Mass Spectrometry v2
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