Paalini Sathiyaseelan

4 Questions 2 Answers 0 Followers

Questions related from Paalini Sathiyaseelan

What does negative mole percent excess/enrichment (MPE) mean?

To understand how glucose is being metabolized in our cell conditions, 13C Glucose was used as a tracer. To determine the carbon contribution from tracer glucose in each metabolite, the MPE was...

21 January 2022 6,522 1 View

Why do the fibroblast cells look "torn" under TEM?

Hi everyone, I recently did a TEM on fibroblast cells and noticed that the cells, including control (normal and untreated)cells appear torn (or massive vacoules). The fixing and processing of the...

21 November 2018 3,763 1 View

Can anyone recommend a lentiviral vector with both a Tet-On promoter and a CMV promoter?

I am trying to design a CRISPR KO line for my gene of interest but the problem is that removing my protein of interest seems to kill the cells. I need a vector that has a Tet-On promoter, so that...

14 December 2016 2,345 3 View

Has anyone exposed cells to Bafilomycin A1 longer than 24hrs?

Baf is used to study autophagy but cells are always exposed within the 2-4 hr range. Has anyone tried exposing cells to Baf at a longer time period or are there any literature that explains what...

01 January 1970 424 1 View