I have sequenced my bacterial RNA samples using Illumina Hiseq and have good quality data. I mapped the reads to the reference genome using " Map with BWA-MEM " tool in galaxy. When I try to analyze RNA seq data, using SAM/BAM to count matrix using HTSeq code (Galaxy Version 0.5), an error keeps coming. It says " Error: ('SAM line does not contain at least 11 tab-delimited fields.', 'line 1 of file /mnt/gal ".

Does anyone know the reason for this error to appear? Is there any way that I can fix it? Thank you

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