Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

How can i send the Mandelys reference Library file ???

hi to all my dear colleagues, i need your help concerning finding the proper way to send and prepare the library file of references by Mandely??

06 July 2020 383 5 View

I would like to quantify cytokines from mice brain using ELISA, What is the total protein concentration i suppose to add in each well?

Hi everyone I want ant to use ELISA to quantify tnf a ,il-1b and inf g in Half brain of mice. I was wondering if any one has done this exactly before and can tell me which concentration of...

07 May 2019 7,701 3 View

Does BHK (Baby Hamster kidney) cell line have specific regulations to Enter Egypt?

I need to import BHK cells to Egypt, but I'm not sure if there are any regulations for it to Enter!

26 February 2019 4,814 1 View

What is the different between UPGMA and Neighbor-Joining Methods in the building of the phylogenetic tree?

the methods of building the phylogenetic tree

12 April 2017 1,050 2 View

Can anyone provide me with some papers regarding the nutrient deficiency within the soil in Iran ?

29 January 2017 650 4 View

Vitamin B12 detection & determination?

What is the best method to detection and determine of Vit. B12 from bacteria?

10 November 2016 6,035 3 View

How can you verify if your research work is novel or not?

03 March 2016 9,154 33 View

Can anyone tell me the difference between a normal colon and colon cancer in rodents?

There is a difference between normal and cancerous tissue of the colon of rodents after injection with 1,2- DMH for 15 weeks.

20 March 2014 4,988 1 View

Do you think that electronic education proved its ability to achieve the required goals that have been submitted by universities??

do you think that electronic education proved its ability to achieve the required goals that have been submitted by universities and scientific institutes??? Agree or Disagree ???

01 January 1970 4,678 29 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View