Something about data transformation in statistics?

I was reading the basics about statistics and get stuck on the problem as stated in the link...

03 April 2017 1,674 2 View

How to set the treeing parameter in PhyML after I get the the most appropriate tree from jmodeltest2?

05 June 2016 1,625 0 View

Will the topology of a phylogenetic tree change after removing a number of sequences and re-run the treeing method?

05 June 2016 3,633 5 View

Error message from mothur's cluster command ?

01 February 2016 4,392 2 View

How good is network analysis to study microorganism cooccurance in complex environment such as soil?

I read some papers using network analysis to study the interaction between different groups of microbes in different soils. I have very little knowledge about this type of analysis. How good it...

09 October 2015 9,758 1 View

Does anyone knows what statistical analysis is used for the pairwise comparison in Kruskal-Wallis test, in SPSS version 22?

04 May 2015 7,694 18 View

I am planing to do ANOVA test on my data, but the data are not normally distributed even after transformation (e.g. log, Box-Cox). What should I do?

I have some continuous variables from my experiment with 6 different treatments, and I would like to ANOVA to compare the means. But the data are not normally distributed even after data...

04 May 2015 1,258 12 View

Will PCR amplify efficiently if the primer has one base mismatch at the 3' end?

I am recently using a primer set to amplify some DNA 16S rRNA from soil. One primer has one base pair mismatch at the 3' end. I would like to know how will that one base mis-match at the 3' end...

05 June 2014 5,963 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View