What does genomic DNA exist after extraction and purification?

Dear all, Here is a very fundamental question: what do genomic DNA (e.g. extracted from plant, animal, microbes, and environmental samples) exist in a solution after extraction and purification?...

04 May 2018 3,370 0 View

Something about data transformation in statistics?

I was reading the basics about statistics and get stuck on the problem as stated in the link...

03 April 2017 1,674 2 View

How to set the treeing parameter in PhyML after I get the the most appropriate tree from jmodeltest2?

05 June 2016 1,625 0 View

Will the topology of a phylogenetic tree change after removing a number of sequences and re-run the treeing method?

05 June 2016 3,633 5 View

How good is network analysis to study microorganism cooccurance in complex environment such as soil?

I read some papers using network analysis to study the interaction between different groups of microbes in different soils. I have very little knowledge about this type of analysis. How good it...

09 October 2015 9,758 1 View

Does anyone knows what statistical analysis is used for the pairwise comparison in Kruskal-Wallis test, in SPSS version 22?

04 May 2015 7,694 18 View

I am planing to do ANOVA test on my data, but the data are not normally distributed even after transformation (e.g. log, Box-Cox). What should I do?

I have some continuous variables from my experiment with 6 different treatments, and I would like to ANOVA to compare the means. But the data are not normally distributed even after data...

04 May 2015 1,258 12 View

Will PCR amplify efficiently if the primer has one base mismatch at the 3' end?

I am recently using a primer set to amplify some DNA 16S rRNA from soil. One primer has one base pair mismatch at the 3' end. I would like to know how will that one base mis-match at the 3' end...

05 June 2014 5,963 8 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View