Yiyu Meng

9 Questions 12 Answers 0 Followers

Questions related from Yiyu Meng

What does genomic DNA exist after extraction and purification?

Dear all, Here is a very fundamental question: what do genomic DNA (e.g. extracted from plant, animal, microbes, and environmental samples) exist in a solution after extraction and purification?...

05 May 2018 3,441 0 View

Something about data transformation in statistics?

I was reading the basics about statistics and get stuck on the problem as stated in the link...

04 April 2017 1,744 2 View

How to set the treeing parameter in PhyML after I get the the most appropriate tree from jmodeltest2?

suppose the running of jmodeltest2 come up the best model for my nucleotide alignment is as follow: Best model = 010231+I+G+F f(a) = 0.24 f(c) = 0.41 f(g) = 0.26 f(t) =...

06 June 2016 1,756 0 View

Will the topology of a phylogenetic tree change after removing a number of sequences and re-run the treeing method?

Hello, suppose I have constructed a phylogenetic tree with all OTU sequences and reference sequences. I now want to have a tree containing only the OTU sequences. Is it a good practical to remove...

06 June 2016 3,723 5 View

Error message from mothur's cluster command ?

when I ran the mothur cluster command, I got an error message shown as below. Is this common? If not, any suggestions to fix it? mothur >...

02 February 2016 4,466 2 View

How good is network analysis to study microorganism cooccurance in complex environment such as soil?

I read some papers using network analysis to study the interaction between different groups of microbes in different soils. I have very little knowledge about this type of analysis. How good it...

10 October 2015 9,834 1 View

Does anyone knows what statistical analysis is used for the pairwise comparison in Kruskal-Wallis test, in SPSS version 22?

I found out that there is a handy option in the Kruskal-Wallis test, to do pairwise comparison between groups, in SPSS version 22. Anyone knows what statistics does it use?

05 May 2015 7,771 18 View

I am planing to do ANOVA test on my data, but the data are not normally distributed even after transformation (e.g. log, Box-Cox). What should I do?

I have some continuous variables from my experiment with 6 different treatments, and I would like to ANOVA to compare the means. But the data are not normally distributed even after data...

05 May 2015 1,333 12 View

Will PCR amplify efficiently if the primer has one base mismatch at the 3' end?

I am recently using a primer set to amplify some DNA 16S rRNA from soil. One primer has one base pair mismatch at the 3' end. I would like to know how will that one base mis-match at the 3' end...

06 June 2014 6,035 8 View