I work on microbial production of Silver Nanoparticles . In the step of synthesis of Silver Nanoparticles it was written that The reaction between the supernatant and Ag+ ions was carried out in bright conditions for 24 hours.
so what does bright conditions mean ?
I know that silver nitrate is sensitive for light . I tried to make a solution of Silver nitrate at a concentration of 10-3 and keep it in light for weeks and the color does not change.
I used to cover the flasks ( Bactria supernatant + silver nitrate) during the experiment but the brown color does not appear , but when I keep it in light ,it appears which is the evidence of formation of silver nanoparticles.
My questions are :
Does light effect the formation of silver Nanoparticles ?
which kind of light do I need ; Sun light or room light ? I use close shaker so should I put light inside it ?
** I attached one of the paper I use .
Dear Dina,
The shape and characteristics of pre-formed Ag nanoparticle suspension will be affected when stored in the light. From our experiments Ag NP (citrate capped) suspension changed colour over time from original 'yellow' suspension to green, blue and sometimes red. The colour can be indicative of particle size and is seen to change as the particles grow and eventually precipitate. A brown colour suspension is also indicative of Ag NPs. You will know that you have Ag NPs if you also have absorbance at ~400 nm. Room light is sufficient to cause a reaction of the silver. You will also need plenty of the extra cellular material to react with and stabilize the AgNP suspension.
Sun light can reduce silver nitrate to produce silver nanoparticles.
DON'T PUT FLASK IN PRESENCE OF SUNLIGHT, IT AFFECT AgNP formation
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