What does a binding buffer do in DNA extraction ?

Noor Edan Jarad @Noor_Jarad

12 December 2018 0 7K Report

MOLECULAR DETECTION

Badges
Science method

More Noor Edan Jarad's questions See All

Why heating Elution buffer in DNA extraction ?

DNA extraction

11 December 2018 2,423 0 View

Can anyone explain the difference between cut Off ELISA and standard ELISA ?

02 March 2018 7,512 0 View

What the best molecular method to diagnose Theleria ?

blood parasites

06 July 2017 7,597 3 View

What the importance of real time PCR than the conventional PCR ?

molecular techniques

05 June 2017 6,531 7 View

Why Taenia solium is more dangerous than T saginata in human ?

taeniasis disease in human

05 June 2017 8,210 6 View

What the causes of pernicious type anemia in the case of Diphyllobothrium latum infection ?

fish tape worm

05 June 2017 4,960 4 View

What the type of cyst of Echinococcus parasite?

many type of cyst according to species

05 June 2017 4,579 9 View

What is the economic importance of plant parasite?

general

04 May 2017 5,992 4 View

What is the main drug is used to prevent Malaria?

04 May 2017 8,285 5 View

What is the difference between the concepts of epidemic disease and endemic disease?

04 May 2017 5,306 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View