Hi,
I have an aminosilane layer on glass which has a net negative charge. I'm curious if you think that adding NHS to this layer should cause a permanent blocking of the charged amines? I know that often, NHS-PEGs or other NHS-R groups are used to passivate a surface, but I wondered what just NHS alone would do to the surface? Also, do you think the NHS-passivated surface would have a neutral net charge?
Interesting question.
NHS-PEG or -R contains NHS attached to PEG through an ester bond (so an NHS ester rather than just NHS), thus making the NHS group a good leaving group upon reaction with Amine.
http://www.piercenet.com/method/amine-reactive-crosslinker-chemistry
NHS unless attached to an ester, it cannot react with an amine.
So my answer would be there will be no reaction between amine and NHS, thus no passivation
Thank you for clearing this up. I did not realize the distinction between NHS ester and NHS! I would have wasted a lot of time before realizing this.
Yes, I have seen that NHS-PEG, and thought it would be a better solution but just trying to save money.
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