I have been reading about cell-free protein expression systems. However my colleagues say they do not find many using the commercial PURE system which used recombinant expression elements. Does anyone know why? Is it flawed?
I would like to publish one huge table as Supplementary resources to a forthcoming paper. The R package 'plotly' enables the creation of interactive tables which can be searched, filtered for...
08 September 2018 8,499 8 View
[Please mind that ggplot will not produce tables] I am a novice R user. I have a huge table with contigs annotations which I'd like to present in full as supplementary results. I wanted to make it...
06 July 2018 8,467 3 View
Dear colleagues, We have a Nanodrop model (Nano-300 AllSheng) which allows for scanning samples from 200-800 nm. I was thinking whether it could be used as an alternative to more sophisticate...
31 December 2017 8,166 4 View
I have been separate piperidine alkaloid isomers with ordinary normal phase silica TLC, revealed with Draggendorf's reagent. However now I am attempting some separations using a silver-nitrate...
10 November 2017 5,055 4 View
Given so many metrics available for evaluating the best journals to which one should submit one's research manuscript, I usually find the Acceptance Rate one of the most desirable. Also as to...
01 February 2017 7,087 12 View
I have searched on the website and did not find a link. Someone dropped that question but Mr Beall answered something else. Having the list offline is useful especially when discussing with my...
10 November 2016 3,855 6 View
Dear collagues, This is probably a very naive question but to date I haven't obtained a satisfactory answer. I often must study old papers. I recurrently find these old papers very reticent and...
08 September 2016 2,715 8 View
Hello, Recently I have been faced with an unexpected issue. I bought this DNA/RNA staining reagent Syto-11 which comes in DMSO. I could see the whole solution was solidified near the tube cap,...
04 May 2015 9,833 6 View
I have seen this video from BBC "Fire Ants and Techno-Chaos" which states that fire ants (mainly Solenopsis invicta) will attack electric equipments in the US, causing considerable damage and...
08 September 2014 4,849 4 View
I would need to do some experiments on ant pupae, but I should genotype them before procedures. As such I would be needing some body part for DNA extraction. I know adults do well after one leg...
05 June 2014 5,802 6 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View