Best-suited data repository for a large interactive 'plotly' table, Supplementary to an 'omic' paper?

I would like to publish one huge table as Supplementary resources to a forthcoming paper. The R package 'plotly' enables the creation of interactive tables which can be searched, filtered for...

08 September 2018 8,366 8 View

How can I implement a 'tooltip' hover effect on a large dataset *table* generated with R plotly?

[Please mind that ggplot will not produce tables] I am a novice R user. I have a huge table with contigs annotations which I'd like to present in full as supplementary results. I wanted to make it...

06 July 2018 8,330 3 View

Unusual applications for the scanning function of a Nanodrop?

Dear colleagues, We have a Nanodrop model (Nano-300 AllSheng) which allows for scanning samples from 200-800 nm. I was thinking whether it could be used as an alternative to more sophisticate...

31 December 2017 8,030 4 View

Best reagent to reveal piperidine alkaloids on silver nitrate-impregnated TLC plates?

I have been separate piperidine alkaloid isomers with ordinary normal phase silica TLC, revealed with Draggendorf's reagent. However now I am attempting some separations using a silver-nitrate...

10 November 2017 4,913 4 View

Database for Journal Acceptance Rates?

Given so many metrics available for evaluating the best journals to which one should submit one's research manuscript, I usually find the Acceptance Rate one of the most desirable. Also as to...

01 February 2017 6,873 12 View

Is there a link to download Beall's List of Predatory Journals?

I have searched on the website and did not find a link. Someone dropped that question but Mr Beall answered something else. Having the list offline is useful especially when discussing with my...

10 November 2016 3,717 6 View

Data and methods in old Science papers?

Dear collagues, This is probably a very naive question but to date I haven't obtained a satisfactory answer. I often must study old papers. I recurrently find these old papers very reticent and...

08 September 2016 2,567 8 View

How can I deal with DMSO crystallisation in stock solution?

04 May 2015 9,673 6 View

How often do fire ants attack electrical installations in the US?

I have seen this video from BBC "Fire Ants and Techno-Chaos" which states that fire ants (mainly Solenopsis invicta) will attack electric equipments in the US, causing considerable damage and...

08 September 2014 4,705 4 View

Would anyone perhaps know if an ant pupa would survive a leg or wing amputation?

I would need to do some experiments on ant pupae, but I should genotype them before procedures. As such I would be needing some body part for DNA extraction. I know adults do well after one leg...

05 June 2014 5,649 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View