Hello,
Recently I have been faced with an unexpected issue. I bought this DNA/RNA staining reagent Syto-11 which comes in DMSO. I could see the whole solution was solidified near the tube cap, when held at -20oC. Naturally in wanting to test it I let it to thaw for several hours on ice -- no result, still solid. Thus I left it on the bench at RT for 2h: still all solid. I read online DMSO may crystallise after exposure to low temperatures.
How do I deal with it? I am scared of heating up the stock solution and affecting the dye!.. Anyone had similar issue? Please share some tips. Thanks
DMSO crystallizes at +18C. RT might not be enough to dissolve all crystals. Try 37C, there are hardly any reagent solutions that cannot stand that for a while.
DMSO crystallizes at +18C. RT might not be enough to dissolve all crystals. Try 37C, there are hardly any reagent solutions that cannot stand that for a while.
hi, Eduardo,
What I usually do is put it into 37 degree water-bath. Crystals will dissolve soon. Well, you can also put at RT, it take longer to dissolve. Hope these help.
Best,
zhilian
Great I will try 37oC then and post here what becomes of it. Thanks!
Ponlo a 37°C en un baño María y luego almacénalo a
temperatura ambiental.
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biochemical Techniques
- Blotting Techniques
- Immunoblotting
- Dyes