High Power control for 48V battery pack - High current control?

Hi, I am working on 48V Lithium-Ion battery pack. I am wondering if there is fast MOSFET (or any other technology), to control the current of charging and discharging the battery with high...

20 January 2021 1,717 4 View

GROMACS: Cannot rename checkpoint file; maybe you are out of disk space?

Hi! i am running a simulation using a cluster and I have encounter an error during my nvt equilibration like this: Cannot rename checkpoint file; maybe you are out of disk space? I deleted some...

03 January 2021 3,544 2 View

How can the resistance of paving asphalt be increased to acid rain?

Modification of the rheological properties of asphalt

25 December 2020 7,390 2 View

Can someone please share the matlab code of FBMC/OQAM PAPR reduction with TR and PTS methods?

I am trying to work on reducing the PAPR of the FBMC/OQAM with the TR and PTS methods. for that if someone can share with me one of these matlab codes. my e-mail: [email protected]

19 December 2020 7,764 1 View

Appreciate the quality qualities of cheese ?

How to estimate the acidity of a cheese?

13 December 2020 4,890 4 View

What is the appropriate polymerase to amplify gDNA greater than 10kb?

I am going to clone gDNA fragment > 10 kb into a pCR TOPO vector. I performed the PCR using KOD PLUS Neo polymerase for 3-steps PCR reaction, I am not able to find the specific size. Several an...

03 December 2020 1,939 5 View

How to get the data of the changing the distance between two Pionts with the applied force?

does anybody knows how can i get the change in the distance between the notch nodes (two points showing in the figure ) vs the applied force in every step as a table out put or curve in...

23 November 2020 352 3 View

Why I got low CI in the EBSD?

Hi I got a low confidence index in the EBSED analysis even after days of polishing using 1 to 0.02 um suspension using 1 lb force, the sample was Al/W composite (has intermetsllic interface)...

26 August 2020 3,547 4 View

Hi Is it needed to etch a sample in order to see dislocations? SEM or TEM microscope should be used for that?

hi please I have Al/W sample and I am looking for screw dislocations on the intermetallic surface, my question is: Should the sample be etched? and should I use the SEM or TEM microscopy? what...

06 August 2020 9,703 2 View

How can i increase my validation and test accuracy?

I am using google colab to train a model for sign language recognition using this dataset: https://www.kaggle.com/feronial/turkish-sign-languagefinger-spelling I tried different architecture...

08 May 2020 4,098 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is required for banking institutions to shift towards contemporary marketing practices in the light of Covid-19 pandemic?

Even after the coronavirus passes, digital solutions for banks will have an enduring relevance, as customer behaviours are expected to change coming out of this crisis toward the best, while banks...

12 February 2021 4,948 10 View