too many downstream applications. in our case- purification of exosomal RNA cargo, and either NGS or qPCR

Exosomal miRNA sequencing could be another option.

In addition, exosomes (from the secretome) could be subjected to mass spectrometry analysis to determine the presence or absence of potential biomarkers in the exosomal fraction. However, it must be stated that the integrity of the exosomes must be assessed either through the use of transmission electron microscopy (TEM) or by confirming the absence of cellular contaminations such as mitochondria, ER, Golgi bodies etc.