Dear Suipwksiow,

At the first you have to clear your analyze level and analyte(s) candidate.

The tumor sample may analyze anthropologically, microscopic specifications may assess with specific or general staining.

The tumor sample may assess at the cellular level, so you have to cell extraction techniques

The tumor sample may assess at the molecular level.

So, you may assess in situ methods or extracted methods.

If you want to extract methods, at the first you have to clear your analyte group:

Extracted analyte may be:

DNA (total, nuclear, mitochondrial, viral,...)

RNA (total RNA, mRNA, micro, short, long non coding, ...)

Protein (fibrous protein, globular protein, membrane protein, soluble free,...)

Carbohydrate (polysacharide,...)

small molecule analytes (metabolites in metabolic pathways)

external molecule? toxins, drugs,...

So, finally, before selection the suitable preparation method and determination method, you have to determine your goal and analyte (s) carefully.

Best

Azin