What is "monoclonal" in monoclonal antibody?

Hi, This may be a silly question, but what is monoclonal in monoclonal antibody? I am new to the field of antibody. Let say, if we generate mAb with mouse, does monoclonal mean that it is...

09 October 2018 8,951 4 View

Does the light chain of IgG differ even for the same subclass?

Hi, For mAb, the light chain contains the highly variable region which is responsible for binding to the antigen. Are these regions different for even the same subclass? For example, the...

09 October 2018 5,873 0 View

What is a difference between laboratory frame energy vs center of mass frame energy?

Hi all, Could someone explain the difference between laboratory frame energy vs center of mass frame energy?

09 October 2018 2,762 0 View

Why is laboratory frame energy always higher than the center of mass frame energy during collisions?

Hi all, Why is laboratory frame energy always higher than the center of mass frame energy during collisions? Could you explain it in terms of physics rather than mathematics?

09 October 2018 8,361 0 View

What is gas-phase basicity of acetate anion (how to compare gas-phase basicity of acetate and histidine?)?

Hi, I am trying to compare the gas-phase basicity of acetate vs histidine. Finding the gas phase basicity of histidine was fairly straightforward as it is shown on NIST chemistry webbook....

08 September 2018 3,367 3 View

How can high res MS compromise the sensitivity?

How could high resolution MS compromise the sensitivity (particularly for qTOF instrument)?

08 September 2018 7,650 2 View

What are coefficient of determinations that can be used for both linear and nonlinear fitting?

Hi all, I have a data set that I want to fit a curve. In order to determine which function agrees with my data. I need the coefficient of determination that can indicate how well it fits. But...

07 August 2017 9,688 1 View

Why are insulin and alcohol dehydrogenase used as a client protein in chaperone assay?

Hi all, I have encountered many papers that almost exclusively use insulin, alcohol dehydrogenase and beta-lactalbumin as aggregated client proteins in chaperone assays. Is there a reason why...

04 May 2017 633 1 View

What is really the n value in ITC?

04 May 2017 5,358 7 View

What type of proteins are hard to crystallize?

Hi all, I am not a crystal structural biologist. I have few questions that could be rather technical. I am aware that there are some proteins that are difficult to study using x-ray...

03 April 2017 2,729 11 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View