In mass spectrometry, people keep talking about peak width from gating and diffusion..
what do they mean?
Hi, This may be a silly question, but what is monoclonal in monoclonal antibody? I am new to the field of antibody. Let say, if we generate mAb with mouse, does monoclonal mean that it is...
09 October 2018 9,163 4 View
Hi all, Could someone explain the difference between laboratory frame energy vs center of mass frame energy?
09 October 2018 2,876 0 View
Hi, For mAb, the light chain contains the highly variable region which is responsible for binding to the antigen. Are these regions different for even the same subclass? For example, the...
09 October 2018 5,970 0 View
Hi all, Why is laboratory frame energy always higher than the center of mass frame energy during collisions? Could you explain it in terms of physics rather than mathematics?
09 October 2018 8,487 0 View
Hi, I am trying to compare the gas-phase basicity of acetate vs histidine. Finding the gas phase basicity of histidine was fairly straightforward as it is shown on NIST chemistry webbook....
08 September 2018 3,478 3 View
How could high resolution MS compromise the sensitivity (particularly for qTOF instrument)?
08 September 2018 7,811 2 View
Hi all, I have a data set that I want to fit a curve. In order to determine which function agrees with my data. I need the coefficient of determination that can indicate how well it fits. But...
07 August 2017 9,817 1 View
Hi all, I have encountered many papers that almost exclusively use insulin, alcohol dehydrogenase and beta-lactalbumin as aggregated client proteins in chaperone assays. Is there a reason why...
04 May 2017 734 1 View
Hi all, The n value in ITC is known to be the "binding stoichiometry". But what really is n value ? How is it defined that it is known to represent the binding stoichiometry? Thanks, Cece
04 May 2017 5,589 7 View
Hi all, I am not a crystal structural biologist. I have few questions that could be rather technical. I am aware that there are some proteins that are difficult to study using x-ray...
03 April 2017 2,915 11 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View