Hi,
For mAb, the light chain contains the highly variable region which is responsible for binding to the antigen.
Are these regions different for even the same subclass?
For example, the variable of IgG1 different from each other?
Hi, This may be a silly question, but what is monoclonal in monoclonal antibody? I am new to the field of antibody. Let say, if we generate mAb with mouse, does monoclonal mean that it is...
09 October 2018 9,163 4 View
In mass spectrometry, people keep talking about peak width from gating and diffusion.. what do they mean?
09 October 2018 3,029 0 View
Hi all, Could someone explain the difference between laboratory frame energy vs center of mass frame energy?
09 October 2018 2,876 0 View
Hi all, Why is laboratory frame energy always higher than the center of mass frame energy during collisions? Could you explain it in terms of physics rather than mathematics?
09 October 2018 8,487 0 View
Hi, I am trying to compare the gas-phase basicity of acetate vs histidine. Finding the gas phase basicity of histidine was fairly straightforward as it is shown on NIST chemistry webbook....
08 September 2018 3,478 3 View
How could high resolution MS compromise the sensitivity (particularly for qTOF instrument)?
08 September 2018 7,811 2 View
Hi all, I have a data set that I want to fit a curve. In order to determine which function agrees with my data. I need the coefficient of determination that can indicate how well it fits. But...
07 August 2017 9,817 1 View
Hi all, I have encountered many papers that almost exclusively use insulin, alcohol dehydrogenase and beta-lactalbumin as aggregated client proteins in chaperone assays. Is there a reason why...
04 May 2017 734 1 View
Hi all, The n value in ITC is known to be the "binding stoichiometry". But what really is n value ? How is it defined that it is known to represent the binding stoichiometry? Thanks, Cece
04 May 2017 5,589 7 View
Hi all, I am not a crystal structural biologist. I have few questions that could be rather technical. I am aware that there are some proteins that are difficult to study using x-ray...
03 April 2017 2,915 11 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
Here, I have used Ag/AgCl as reference electrode and 0.5M KOH.
29 July 2024 4,538 3 View
Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...
28 July 2024 6,133 8 View
I used Aqua regia to clean Au nanoparticles in the used glassware. But for some reasons, it is not heating up upon mixing. The color did change from no color to light orange. The Au NPs on the...
25 July 2024 4,390 0 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.
25 July 2024 3,395 3 View
I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....
24 July 2024 4,058 5 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Photo-electric effect A light particle moving towards a surface cannot (by collision) force an electron particle to move away from that surface. A light wave can instead make interference with a...
19 July 2024 8,389 9 View