What is "monoclonal" in monoclonal antibody?

Hi, This may be a silly question, but what is monoclonal in monoclonal antibody? I am new to the field of antibody. Let say, if we generate mAb with mouse, does monoclonal mean that it is...

09 October 2018 8,951 4 View

What do peak width from gating and diffusion mean?

In mass spectrometry, people keep talking about peak width from gating and diffusion.. what do they mean?

09 October 2018 2,890 0 View

Does the light chain of IgG differ even for the same subclass?

Hi, For mAb, the light chain contains the highly variable region which is responsible for binding to the antigen. Are these regions different for even the same subclass? For example, the...

09 October 2018 5,873 0 View

What is a difference between laboratory frame energy vs center of mass frame energy?

Hi all, Could someone explain the difference between laboratory frame energy vs center of mass frame energy?

09 October 2018 2,762 0 View

Why is laboratory frame energy always higher than the center of mass frame energy during collisions?

Hi all, Why is laboratory frame energy always higher than the center of mass frame energy during collisions? Could you explain it in terms of physics rather than mathematics?

09 October 2018 8,361 0 View

What is gas-phase basicity of acetate anion (how to compare gas-phase basicity of acetate and histidine?)?

Hi, I am trying to compare the gas-phase basicity of acetate vs histidine. Finding the gas phase basicity of histidine was fairly straightforward as it is shown on NIST chemistry webbook....

08 September 2018 3,367 3 View

What are coefficient of determinations that can be used for both linear and nonlinear fitting?

Hi all, I have a data set that I want to fit a curve. In order to determine which function agrees with my data. I need the coefficient of determination that can indicate how well it fits. But...

07 August 2017 9,688 1 View

Why are insulin and alcohol dehydrogenase used as a client protein in chaperone assay?

Hi all, I have encountered many papers that almost exclusively use insulin, alcohol dehydrogenase and beta-lactalbumin as aggregated client proteins in chaperone assays. Is there a reason why...

04 May 2017 633 1 View

What is really the n value in ITC?

04 May 2017 5,358 7 View

What type of proteins are hard to crystallize?

Hi all, I am not a crystal structural biologist. I have few questions that could be rather technical. I am aware that there are some proteins that are difficult to study using x-ray...

03 April 2017 2,729 11 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

Did any urologists have experienced with retroperitonel leiomyosarcoma from renal vein with IVC enxtension?

Recently, we got one lady with right side retroperitoneal mass involving renal vein and extends into IVC upto diaphragm. CT suggested leiomyosarcoma. Right kidney separately seen from mass and it...

19 February 2021 6,873 1 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View