I am trying to carryout diagnostic digest with AatII and DraIII (Adei) but the two did not cut as expected. Could it be because of CpG sensitivity?
The tests are the paired lanes separated by blank from left to right. The first lane on the left is treated with enzyme (Bam HI, AatII, Xbal, Dra III). The second lane contains the uncut plasmid in each case.
These are not PcR products, they are plasmids from miniprep.
I think you'll need to explain a little bit more. Is lane 1 showing two cleaved products?
Was your DNA bisulphite treated prior to the PCR?
I am trying to carryout diagnostic digest with AatII and DraIII (Adei) but the two did not cut as expected. Could it be because of CpG sensitivity?
- In case of AatII, it is possible.
According to the New England biolabs website the methylation sensitivity of DRAiii is
dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Impaired by Overlapping
and the sensitivity of Aatii is
dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked
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