Kehinde Oyeniran

6 Questions 10 Answers 0 Followers

Questions related from Kehinde Oyeniran

Agrobacterium tumefaciens C58Ci?

I have been trying to make this A. tumefaciens c58ci from last year glycerol stocks stored at -80C competent.I firstly grew in LB medium supplemented with rifampicin and gentamicin. One thing I...

06 June 2018 6,250 3 View

Cutting a dimer insert?

I already dimerized an insert in a vector and now trying to cut the dimer for further cloning; since there are no suitable clean ways to this, I intend to do a full xbal digest of the vector (It...

05 May 2018 1,337 6 View

Gel Purification and Ligation?

Can I gel purify two bands for ligation in a tube? Do you think I should extract in different tubes?

03 March 2018 5,944 0 View

What do i need to worry about if a restriction enzyme is CpG methylation sensitive? Is there any way to make it work?

I am trying to carryout diagnostic digest with AatII and DraIII (Adei) but the two did not cut as expected. Could it be because of CpG sensitivity? The tests are the paired lanes separated by...

10 October 2017 2,423 6 View

How long can it take to self ligate a DNA band of interest to form a dimer?

In order to make an infectious clone of geminivirus

09 September 2017 8,844 0 View

After running sequence alignment; how do I look for evidence of evolution within the sequences?

For bioinformatics

09 September 2017 1,305 2 View